目的利用微波提取技术同时从甘草中提取甘草酸和甘草黄酮。方法考察了影响提取率的因素,包括提取溶剂、液固比、微波时间和功率等。结果确定了微波提取甘草有效成分的最佳工艺:70%乙醇为提取溶剂,按10∶1(mL/g)的液固比,微波中高火辐照4 m in,提取3次,甘草酸的提取率为3.06%,甘草黄酮提取率为3.00%,与热回流法提取4 h的结果接近。结论该提取工艺既缩短了提取时间,又提高了甘草药材的综合利用率。
Production of lipase by Rhizopus arrhizus was studied in a rotating biological contactor(RBC)with polyurethane foam(PUF),as a porous biomass support,attached on both sides of the RBC disks.The effects of aeration rate,rotating rate of discs,volume of broth and the number of discs on the lipase activity were studied in the 6 L RBC,and the optimum fermentation condition was as follows:4 L·min-1 aeration rate,100 r·min-1 rotating rate,3.5 L medium with three discs in 6 L RBC.Repeated batch fermentation by immobilized mycelium was tested and it was found that immobilized cells showed high stability for repeated use.Five repeated batches could be carried out in 6 L RBC and the lipase productivity increased from 3125 U·h-1 in batch fermentation to 9512 U·h-1 in repeated batch fermentation,which was 3 times as high as that in batch fermentation.
