In order to reveal the physiological reaction and adaptability of natural Sabina vulgaris community to sandy environment in different successional stages, we determined physiological index of drought_resistance of S.vulgaris under different successional stages by colorimetry in TuKe of Mu Us Sandland. The results showed that with the process of the succession, PRO and MDA contents increase first, and then decrease during the process of the succession. The variance analysis showed that the difference of the PRO contents among stages is more significant. The difference of the SOD and POD activity is more significant among stages, but the CAT activity has no significant change. In the fourth successional stage, the contents of MDA and PRO increase, the activity of SOD and POD increase. All of them show the physiological characters of damaged plant under the environment hardness. The correlation analysis between PRO、MDA and protective enzymes activity showed: the positive correlation of the change of SOD、POD activity with the change of MDA contents is significant. The decrease of MDA will increase protective enzymes activity which in the leaf. The response of the physiological index to the environment hardness in different successional stage is not similar. First, some damaged characters appeared in the fourth stage; second, some index are stable in the third stage. It indicated that the third stage is a stable period.