This paper presents the study of a three-dimensional(3D) isolation system.Firstly,the authors investigated the effects of an innovative 3D isolator,which was composed of a connecting plate,a rubber pad for vibration isolation in the vertical direction and a horizontal rubber bearing for seismic isolation in both horizontal directions.Secondly,the authors designed such a vibration isolation system and installed it underneath two specific residential buildings which were built directly over an existing subway communication hub platform in Beijing.These buildings required good performance vibration and seismic isolation system to reduce the impact from the running of nearby subway trains.Finally,in situ tests were conducted for both the isolated and the non-isolated buildings for the purpose of comparison.The test results showed that the maximum acceleration response level of the isolated superstructure is reduced by 10% as compared to that of the platform.The maximum attenuation of vibration reaches up to 25 dB.The 3D system explored in this paper is very effective in control and suppression of building vibration induced by earthquakes or running of trains.