利用秸秆产沼气是秸秆无害化处理解决环境污染问题的可行手段。本研究针对秸秆特殊结构而导致产沼气少、启动慢、利用率低等问题,采用自培养漆酶预处理水稻秸秆,提高了秸秆产气率及利用率。通过研究发现,当漆酶添加量为40 m L、发酵温度为35℃、预处理7 d的条件下,秸秆的产气量最高;同时发现,金属离子的添加对沼气产量有很大影响,其中Cu2+添加后产气量提高最多。另外,研究发现预处理天数为24 d的秸秆木质素纤维素降解率最高。
White rot fungi were optimized to cultivate highly active laccase. The characteristics of laccase incubated by continuous culture were compared with those of direct culture. The enzyme activity of laccase incubated by continuous culture technology reached a higher value on the fifth day of the growth. The optimization incubation time of high activity laccase was the eleventh day. A large amount of highly active laccase can be obtained in a relatively short time by continuous culture to replace traditional laccase. After laccase treatment, the lignin composition of wood fibers were oxidation-catalyzed by laccase. The number of chemical-bonding points between the wood fibers was increased. The wood fibers treated by laccase were fabricated into boards and their mechanical properties improved with the laccase-incubation times. Compared with the fiberboards made from fibers that were pre-treated by laccase of incubation 5 days, the static bending strength of those that were pre-treated by laccase of incubation 11 days was increased by 18.95%, the elastic modulus was increased by 35.49%, and the internal bond strength was increased by 44.11%.
