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国家重点基础研究发展计划(2005CB522504)

作品数:1 被引量:16H指数:1
相关作者:吴清玉柳德斌张宏山张阳周玉祥更多>>
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Conditional Gene Inactivation Reveals a Crucial Role for zgc:112094 in Zebrafish Development
We performed a pilot screen for gene trap in zebrafish using a Tol2 based construct that contains a unidirecti...
梁巍
关键词:ZEBRAFISH
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反转录病毒介导的斑马鱼全基因组高效突变
我们发展了一套高效的斑马鱼反转录病毒插入突变技术。通过在斑马鱼胚胎中注射源于鼠莫勒氏白血病病毒的假型反转录病毒和 LM-PCR 技术定位病毒插入的位点等方法,我们可以得到大量携带有反转录病毒插入的斑马鱼。通过对~3000...
郑乃中王冬梅Li-En JaoKyle DolanJessica IveySeth ZoniesXiaolin Wu邬康迈杨红波孟庆超朱作言Shawn M.Burgess林硕张博
关键词:斑马鱼反转录病毒
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pku301/Ets Like is Required for Development of Serotonergic Neurons and β Cells in Zebrafish
Pancreas is a vertebrate specific internal organ and consists of exocrine and endocrine compartments.The endoc...
Lin-Jie Xu~1,Wei Gao~1,Fei Qi~1,Zhi Jiang~1,Zuo-Yan Zhu~1,Bo Zhang~1,Shuo Lin~(1,2) 1 Key Laboratory of Cell Proliferation and Differentiation,Center of Developmental Biology and Genetics,College of Life Sciences,Peking University,Ministry of Education,Beijing 100871 2 Department of Molecular,Cell & Developmental Biology,University of California,Los Angeles, Los Angeles,CA 90095-1606,USA
关键词:ETSPANCREASZEBRAFISH
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小鼠心肌组织3种总RNA提取方法比较被引量:16
2010年
目的:探索一种高效的心肌组织总RNA提取方法,对以后总RNA提取工作进行指导。方法:取小鼠心肌组织,分别采用剪刀剪碎法(剪碎法)、研钵加液氮研磨法(研磨法)、离心柱法(过柱法)对小鼠心肌组织进行总RNA提取,紫外分光光度计定量,聚丙烯酰胺凝胶电泳初步定性。结果与结论:研磨法提取出的总RNA量大,质量可靠,较适合于日常总RNA提取;剪碎法对于一些实验条件较差的地区仍然是一种可取的方法;过柱法对于小量心肌组织的提取较为适合。3种方法均能提取出总RNA。
张宏山许明张阳柳德斌吴清玉周玉祥
关键词:心肌组织RNA提取
IF-Like Protein Ngs is Essential for Maintenance of Chordocytes in Zebrafish
The notochord is a characteristic structure for all members of the phylum Chordata, which plays critical roles...
夏梽丹Helena Telfer
关键词:ZEBRAFISHNOTOCHORD
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Identification of Novel Genes Related to Embryonic Vascular Development Using To12 Transposon Mediated Enhancer Trap in Zebrafish
Cardiovasculature forms during evolution and arises from very early stage during embryonic development.Blood v...
Yu-Lin Xue~1,An Xiao~1,Yue Jia~1,Yue Gao~1,Zuo-Yan Zhu~1,Bo Zhang~1,Shuo Lin~(1,2) 1 Key Laboratory of Cell Proliferation and Differentiation,Center of Developmental Biology and Genetics,College of Life Sciences,Peking University,Ministry of Education,Beijing 100871 2 Department of Molecular,Cell,and Developmental Biology,University of California,Los Angeles, Los Angeles,CA 90095-1606,USA
关键词:ZEBRAFISHTRANSGENIC
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Genomic Deletion Induced by To12 Transposon Excision in Zebrafish
Imprecise excision of P element is widely used in Drosophila to disrupt nearby genes.However,this is the only ...
Chi-Yen Tam
关键词:ZEBRAFISHTRANSPOSONDELETION
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Retroviral Insertional Mutagenesis Screen for Genes Involved in Human Disease Modeling in Zebrafish
Through retrovirus insertion based mutagenesis screen in zebrafish(Danio reiro) we have obtained several inser...
Ying Zhang~1,Nai-Zhong Zheng~1,Shuo Lin~(1,2),Bo Zhang~1 1 Key Laboratory of Cell Proliferation and Differentiation,Center of Developmental Biology and Genetics,College of Life Sciences,Peking University,Ministry of Education,Beijing 2 Department of Molecular,Cell & Developmental Biology,University of California,Los Angeles, Los Angeles,CA 90095-1606,USA
关键词:ZEBRAFISHRETROVIRUS
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