生物碱与一些特殊的试剂作用生成沉淀。这些反应可用于生物碱的检测和精制。本文分别在同步辐射装置和微聚焦 X 射线成像系统上用 X 射线相衬成像(XPCI)方法研究了三种不同类型的生物碱沉淀的结构。研究结果显示:在 Hager 试剂作用下,有机胺类的秋水仙碱沉淀呈针状,吡啶类的槟榔碱沉淀呈胶团状,异喹啉类的小檗碱沉淀呈花瓣状。与目测或在光学显微镜下观察到的形态有很大差别。为今后用 XPCI 方法研究更复杂的化学反应体系提供参考和依据。
Objective: Phase-contrast X-ray imaging which reduces radiation exposure, is a promising technique for observing the inner structures of biological soft tissues without the aid of contrast agents. The present study intends to depict blood vessels of rabbits and human livers with hard X-ray in-line outline imaging without contrast agents using synchrotron radiation. Methods: All samples were fixed with formalin and sliced into 6 mm sections. The imaging experiments were performed with Fuji-IX80 films on the 4W1A light beam of the first generation synchrotron radiation in Beijing, China. The device of the experiment, which supplies a maximum light spot size of 20×10 mm was similar to that of in-line holography. The photon energy was set at 8 KeV and high quality imagines were obtained by altering the distance between the sample and the film. Results: The trees of rabbit-liver blood vessels and the curved vessels of the cirrhotic human liver were revealed on the images, where vessels < 20 μm in diameter were differentiated. Conclusion: These results show that the blood vessels of liver samples can be revealed by using hard X-ray in-line outline imaging with the first generation synchrotron radiation without contrast agents.
An experimental study on nondestructive X-ray imaging of inner structure of soft tissues in phase con-trast has been conducted with Beijing Synchrotron Radiation Facility (BSRF). Modification to the beamline setupwas made to enlarge the X-ray beam section and consequently larger samples could be imaged. In-line setup was em-ployed for experiments. Results on a series of samples were given and soft-tissue details of less than 50 μm inside afresh goldfish were obtained. Diagnosis of tumor in its early stage was also investigated taking SD rats as the model.Tumor at the size of ～ 100μm was observed. Potential of this technique in clinic diagnosis was discussed.
