目的:观察腹外侧眶皮层(ventrolateral orbital cortex,VLO)内微量注射谷氨酸对大鼠后爪注射福尔马林诱发的腰段脊髓背角Fos表达的抑制效应以及该效应是否通过中脑导水管周围灰质(PAG)下行抑制系统介导。方法:免疫组织化学染色技术。结果:(1)VLO内微量注射谷氨酸(100nmol/0.5μl)明显抑制大鼠后爪注射福尔马林诱发的脊髓背角Fos表达,与注射生理盐水相比,差异显著(P<0.001),并且这种抑制效应可被VLO内预先注射非选择性谷氨酸受体拮抗剂kynurenic acid(2nmol/0.5μl)所翻转,与单独注射谷氨酸相比差异显著(P<0.001),但与注射生理盐水相比无显著差异(P>0.05);(2)双侧腹外侧PAG内微量注射局麻剂利多卡因(0.2nmol/0.5μl)可以明显阻断VLO内微量注射谷氨酸(100nmol/0.5μl)对大鼠脊髓背角Fos表达的抑制效应,与单独注射谷氨酸相比差异显著(P<0.001),而双侧腹外侧PAG内微量注射生理盐水不影响VLO内微量注射谷氨酸对大鼠脊髓背角Fos表达的抑制效应(P>0.05)。结论:VLO内谷氨酸可能通过其受体激活PAG脑干下行抑制系统在脊髓水平抗炎性持续性伤害感受效应。
Objective Previous studies have shown that the ventrolateral orbital cortex(VLO)is part of the endogenous anal...
Yan ZHAO~2,Wen-Jin XU~1,Feng-Yi XU~1,Fu-Quan HUO~(1,*),Chun-Xia YAN~2,Jing-Shi TANG~1 1 Department of Physiology,2 Department of Forensic Medicine,Xi’an Jiaotong University School of Medicine,Xi’an, Shaanxi 710061,China
Previous studies have indicated that the ventrolateral orbital cortex(VLO),as a high center,is involved in an ...
Feng-Yi XU,Fu-Quan HUO~*,Chao-Ling QU,Jing-Shi TANG Department of Physiology and Pathophysiology,Key Laboratory of Environment and Genes Related to Diseases,Ministry of Education,Xi’an Jiaotong University School of Medicine,Xi’an 710061,China
