At the moment,little is found on the whole genomes of spiders in public databases.To this end,this study aimed to analyze the morphology,biological characteristics,and genome of Pardosa pseudoannulata,which was expected to add genome information of spiders.To be specific,Illumina sequencing was used to sequence the genome of this spider species,and K-mer(K=17)analysis to estimate the size,heterozygosity,and repetitiveness of the genome.More than 142.11 Gb high-quality reads were obtained,which were assembled into 3508.13 Mb contigs(N50=1035 bp)and 3542.56 Mb scaffolds(N50=1258 bp).Two peaks were found in the 17-mer distribution map.The genome of P.pseudoannulata was 4696.86 Mb,with the heterozygosity of 0.21%and 84.32%of repetitive sequences.This indicated that repetitive sequences were highly abundant in the genome of P.pseudoannulata and multi-generation selfing before genome sequencing can reduce the repetitiveness.In addition,NOVOheter can be used for genome assembly.This study layed a basis for the publication of spider genome data and provided a reference for the research on spider genomics.
Blooms of Microcystis aeruginosa occur frequently in many freshwater ecosystems around the world,but the mechanism of recovery has not been fully understood.In our previous study,three benthic bacterial species(E.sp013,Ba.spD06,and Ba.spD24)were identifi ed capable of promoting the recruitment of M.aeruginosa.Here,we further investigated the transcriptional response of M.aeruginosa to the benthic bacteria in early phase of recruitment by means of RNA-Seq analysis.In total,5803803 unigenes on average length of 404 bp were obtained from the transcriptome of M.aeruginosa.There were 54982 unigenes identifi ed as benthic bacteria-responsive unigenes based on the expression level analysis.Results of the protein-protein interaction analysis(PPI)show that the hub genes of the benthic bacteria responsive unigenes mediated network were ribosomal proteins of 30S and 50S,and the most signifi cant functional module of the network was related to the ribosome.Both the unigenes encoding the translation initiation factors(IF-2,IF-3)and elongation factors(lepA,fusA,and tufA)were up-regulated to respond benthic bacteria.Therefore,it indicates that the benthic bacteria have a positive infl uence on activating the ribosome during the early recovery stage of M.aeruginosa.
Juan WANGYuande PENGZhi WANGWansheng ZOUXianjin PENGQisheng SONG
