We have developed a rapid microarray-based assay for the reliable detection of H5, H7 and H9 subtypes of avian influenza virus （AIV）. The strains used in the experiment were A/Goose/Guangdong/1/96 （H5N1）, A/African starling/983/79 （H7N1） and A/Turkey/Wiscosin/1/66 （H9N2）. The capture DNAs clones which encoding approximate 500-bp avian influenza virus gene fragments obtained by RT-PCR, were spotted on a slide-bound microarray. Cy5-labeled fluorescent cDNAs, which generated from virus RNA during reverse transcription were hybridized to these capture DNAs. These capture DNAs contained multiple fragments of the hemagglutinin and matrix protein genes of AIV respectively, for subtyping and typing AIV. The arrays were scanned to determine the probe binding sites. The hybridization pattern agreed approximately with the known grid location of each target. The results show that DNA microarray technology provides a useful diagnostic method for AIV.
WANG Xiu-rong YU Kang-zhen DENG Guo-hua SHI Rui LIU Li-ling QIAO Chuan-ling BAO Hong-mei KONG Xian-gang CHEN Hua-lan
Background Pulmonary-vein isolation （PVI） is currently used for the treatment of chronic and paroxysmal atrial fibrillation and a major risk of PVI is thromboembolism. The purpose of this study was to observe embolic event rate in patients with persistent or paroxysmal atrial fibrillation （AF） undergone PVI. Methods Circumferential PVI （CPVI） was performed in 64 consecutive patients with persistent AF （42 men, aged （60.0 ±9.1） years） and in 84 consecutive patients with paroxysmal AF （53 men, aged （61.4±9.3） years）. Warfarin was administrated in all patients before ablation for at least 3 weeks （（5.2±2.6） weeks） and continued for at least 3 months post ablation with international normalized ratio （INR） of 2.0-3.0. During CPVI, intravenous heparin was given at a dose of 5000-8000 U or 75-100 U/kg, followed by 1000 U or 12 U/kg per hour. Results In patients with persistent AF, 1 patient developed embolic event during ablation and 3 patients developed embolic events after ablation. In contrast, no thromboembolic event was observed in patients with paroxysmal AF （4/64 vs 0/84, P=-0. 033）. Conclusion Thromboembolic event rate related to CPVl is significantly higher in patients with persistent AF than that in patients with paroxysmal AF.
ZHOU Jing-min LIU Shao-wen LIN Jia-xiong NIE Zhen-ning WU Hong-yi ZHOU Jun HAO Ying CAI Nai-sheng GE Jun-bo
