AIM: To investigate the cytotoxicity, anti-inflammatory activity, and action mechanism of root bark extracts of Acanthopanax henryi. METHOD: The hot methanol extract of the root bark of A. henryi was subjected to XAD-4 column chromatography eluting with a gradient of methanol in water. The cytotoxicity and anti-inflammatory effects of the MeOH fractions were evaluated on the inhibition on lipopolysaccharide(LPS)-induced nitric oxide, prostaglandin E2, interleukin-1β, and interleukin-6 production in RAW 264.7 macrophages. RESULTS: The 80% MeOH fraction was a better inhibitor of LPS-induced NO, PGE2, IL-1β, and IL-6 production, and expression of inducible nitric oxide synthase(iNOS) at the protein levels in a concentration-dependent manner. CONCLUSION: The 80% MeOH fraction of A. henryi root bark has significant anti-inflammatory activity. This provides a pharmacological basis for clinical application for the treatment of inflammation.
KIM Jong-HwanLIU Xiang-QianDAI LingYOOK Chang-SooLEE Kyung-Tae
To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax graeilistylus W. W. Smith （AGS）, the crystal structure was measured with a Bruker APEX-Ⅱ area-detector diffractometer instrument and the technology of extracting in combination hydrolysis in situ （ECHS） was compared with these of traditional methods. The crystal belongs to the monoclinic system, space group P2b with unit cell parameters： a=（8.3652±0.0006） nm, b=（24.721±0.002） nm, and c=（14.5587±0.0011） nm, α=90°, β=97.850 （4） °, γ=90 °, V=2982.51 nm3, Dc= 1.179 mg/m3, and the molecular number （Z） of elementary structures was 2. The comparisons show that the extraction rate of acankoreanogenin with ECHS methods is much higher than that of traditional methods. Then, central composite design-response surface methodology （CCD-RSM） was adopted for optimizing the extraction rate of ECHS methods. The optimized values of extraction parameters are as follows： for the for extraction process of acid hydrolysis are that extraction time 110.8 min, solvent-herb ratio 11.5 and acid content 5.25%; the best extraction process of basic hydrolysis are that extract time 120 min, solvent-herb ratio 8.7 and the alkali content 8.79%. Finally, the extracts were purified with decolorizing carbon after alkali solution and acid-isolation and purity of acankoreanogenin was 98.7%.
