Objective:We explored the role and molecular mechanism of Microcystin-LR(MC-LR) in hepatocarcinogenesis.Methods:The two-stage-medium-term theory was applied to the establishment of the animal model.The promoting effect of MC-LR on liver tumor was evaluated with the Albert γ-GT methods.During the tumor-promoting course,the effects of MC-LR on the regulation and expression of the Bcl-2 and Bax genes were studied with immunohistochemical technique and RT-PCR.Results:MCLR could enhance the positive reaction of Albert γ-GT(GGT),a preneoplasm marker.The positive reaction rate of GGT in diethylnitrosamine(DEN) + MC group was significantly higher than that in the DEN control group.The protein and RNA expression of Bcl-2 was significantly increased by MC-LR,and that the protein expression of Bax was decreased simultaneously.Conclusion:These results indicate that the MC-LR can inhabit apoptosis through the Bcl-2 and Bax genes.Therefore,we conclude that the expression change of Bcl-2 and Bax genes possibly plays an important role in the promotion of liver tumor by MC-LR.
Zhijian HuHua ChenJinfa XueXiaoyang ZhangXishun ShiHong Zou
