The research, based on pol ens of Zygocactus truncates during ful-bloom period, selected suitable culture medium and temperature for pol en germination and explored the effects of storage temperatures and pol en col ection methods on pol en vitality of Zygocactus truncates and the results showed that the culture medium containing 200 g/L saccharose, 20 mg/L boric acid, and 20 mg/L calcium chloride is suitable for pol en germination of Zygocactus truncates; when the temperature kept below 20 ℃, pol en germination period last short, and germination rate kept higher;pol en germination performed better with filaments, and pol en tube grew wel .