The silica opal templates were prepared from three silica colloids of different diameters of 230 nm, 500 nm and 1.5 mm by a filtration route. The large-scale stable opal template membranes after sintering the deposited SiO2 opal template can be successfully obtained by optimizing the pH value and NaCl concentration in silica colloidal solutions. The three-dimensionally ordered macroporous（3DOM） polyimide membranes without crack were fabricated by reproducing the structure of silica opal template. We prepared the pore-filling composite proton exchange membranes by filling the 3DOM structure with proton conducting organosilane sol. The result indicates that the composite membranes exhibit higher water uptake than pure filling organosilane gel. The proton conductivity increased with the increasing of pore cell in composite membranes.
The development of nanotechnology provides a new method for genetic engineering.However,the nanoparticles as gene carriers have been mainly used in the mammalian cells so far.We observed that ZnS nanoparticles modified with positively charged poly-L-lysine（PLL） successfully delivered GUS-encoding plasmid DNA into tobacco cells by means of ultrasound-assisted method.Polymerase chain reaction（PCR） detection,Southern blot analysis and GUS histochemical staining were carried out for the regenerated plants.The stable genetic modified plants mediated by ZnS nanoparticles can be obtained.This article demonstrates the great potential of nanoparticles as gene carrier in plant transformation and proves a novel approach for plant genetic decoration.
FU Yu-qinLI Lu-huaWANG Pi-wuQU JingFU Yong-pingWANG HuiSUN Jing-ranLÜ Chang-li
Although nanotechnology is considered to be one of the most important technologies to promote social and economic development in the twenty-first century, its application in agriculture is relatively few compared with those in other fields. In this article, plasmid carrying GUS gene was successfully delivered into tobacco（Nicotiana tabacum） by mesoporous silica nanoparticles（MSNs） modified with positively charged poly-L-lysine（PLL） with the assist of ultrasonic method. Stable transformation was confirmed by polymerase chain reaction（PCR） detection and GUS histochemical staining. Meanwhile, we also studied the factors that could enhance the genetic transformation efficiency. The result suggests that the callus receptor and a suitable DNA/MSNs ratio contribute a lot to the transformation efficiency. In a word, our research provides an efficient and cost-effective method for gene delivery to plant.
WANG ZhongniLIU HuijingLI LuhuaLI QuanliangWANG XiuranJIANG YuanFU YuqinLU Changli
