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重庆市自然科学基金(2006BB5403)

作品数:2 被引量:5H指数:2
相关作者:温海霞蔡家利余陆隆张明明兴加更多>>
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不同叶型半夏蛋白质含量测定被引量:3
2009年
目的研究并比较不同叶型半夏蛋白质含量。方法采用分光光度计测定药材中蛋白质含量。结果不同生境条件下,芍药叶型半夏间蛋白质含量差异明显,桃叶型半夏蛋白质含量差异不明显;同一生境条件下,芍药叶型半夏蛋白质含量高于桃叶型。结论半夏栽培生产中,应根据栽培生产需要提高种子纯度。
温海霞张明蔡家利明兴加余陆隆
关键词:半夏叶型蛋白质
Preliminary analysis of the protein contents in Pinellia ternata with different types of leaves被引量:2
2009年
Total proteins extracted from Pinellia ternata with different types of leaves have been assayed by UV absorbance, blue native polyacrylamide gel electrophoresis (BN-PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE). We found that the total protein contents of P. ternata with the herbaceous peony and claw types of leaves were much higher than that with other types of leaves. P. ternata collected from Nanchuan County, except the ones with herbaceous peony leaves, displayed a high similarity with each other. Both UV absorbance and cluster analysis indicated that the protein content in P. ternata with the herbaceous peony leaves was less affected by habitats than that with other types of leaves. The results showed that it was necessary to increase the homogeneity of seeds for improving the protein content ofP. ternata.
温海霞蔡家利张明明兴加刘日勇唐偲雨周卿瑞
关键词:PROTEINVARIATIONPAGE
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