The engineering application of natural hyperaccumulators In removing or inactivating metal pollutants from soil and surface water In field trials mostly presents the insurmountable shortcoming of low efficiency owing to their little biomass and slow growth. Based on further understanding of the molecular mechanism of metal uptake, translocation, and also the separation, identification, and cloning of some related functional genes, this article highlights and summarizes In detail the advances in research on transgenlc techniques, such as Agrobacterlurn tumefaciens-medlated transformation and particle bombardment, in breeding of plants for metal resistance and accumulation, and points out that deepening the development of transgenlc plants Is one of the efficient approaches to improving phytoremedlatlon efficiency of metalcontaminated environments. From the viewpoint of sustainable development, governments should strengthen support to the development of genetic engineering for metal resistance and accumulation In plants.
Ri-Qing ZhangChun-Fang TangShi-Zhi WenYun-Guo LiuKe-Lin Li
为了加快油茶品种改良,提高油茶花药愈伤组织的诱导效率,在低温预处理(4℃)的基础上,以M S为基本培养基,研究了不同浓度的2,4-D、NAA及2,4-D、6-BA配比对普通油茶花药愈伤组织诱导效率的影响。结果表明:2,4-D、NAA诱导普通油茶愈伤组织的较优组合为2,4-D 0.6 m g/L,NAA 0.05或0.5 m g/L,愈伤组织诱导率分别达到35.56%和24.65%,在油茶花药愈伤组织诱导过程中,2,4-D有利于愈伤组织的形成,是最重要的因素;2,4-D、6-BA的混合使用更有利于愈伤组织的发生,并且两种激素适当的配比是提高愈伤组织诱导率的重要因素,0.5 m g/L的2,4-D与0.2 m g/L 6-BA组合是实验得出的较优水平,愈伤组织诱导率达到42.74%。
