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国家自然科学基金(30960256)

作品数:6 被引量:40H指数:3
相关作者:蒋慧马金萍应璐马春晖张玲更多>>
相关机构:塔里木大学云南农业大学更多>>
发文基金:国家自然科学基金公益性行业科研专项国家科技支撑计划更多>>
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Biological Characteristics and Rumen Microorganisms of Gayal(Bos frontalis)in Yunnan Province被引量:3
2011年
[Objective]The paper was to provide reference for further study and development of gayal(Bos frontalis).[Method]According to the research status at home and abroad,the biological characteristics and rumen microorganisms of gayal in Yunnan Province was studied.[Result]Gayal in Yunnan had typical body form and very good meat production performance,its muscle fiber diameter was significantly less than other kinds of cattle;the water holding ratio,muscle tenderness and muscle succulency were significantly higher than others;its amount,shape and structure of chromosome were different from yellow cattle(Bos taurus)and wild cattle(Bos gaurus),and the amounts of those chromosomes(2n)were 58,60 and 56,respectively.It could create hybrid with yellow cattle;the gayal's special diet was bamboo,its in vitro dry matter digestibility(IVDMD)on various crude forage was significantly higher than yellow cattle in Yunnan;the viable bacteria and cellulolytic bacteria in rumen were 4.51×109 and 1.63×109 CFU/ml,which was significantly higher than yellow cattle in Yunnan,its dominant bacteria in rumen mainly was cellulolytic bacteria.[Conclusion]Gayal not only had high academic value,but also had a great development value.
杨舒黎苟潇冷静毛华明邓卫东吴锡川
骆驼刺源产乳酸的枯草芽孢杆菌分离鉴定和菌株特性研究
2016年
为了解疏叶骆驼刺青贮中乳酸菌的组成,用MRS培养基对骆驼刺青贮中的细菌进行分离和纯化,通过革兰氏染色、细胞形态学观察、生理生化试验和16SrDNA序列分析对分离的单菌落进行菌株特性研究和种属鉴定。结果表明:L14菌株呈杆状,G^+,有芽孢;产乳酸,液化明胶,水解淀粉,触酶阳性,温度生长范围15-55℃,酸生长范围pH值为4-7,NaCl生长范围为2%-10%,可利用多种碳源和氮源;16SrDNA测序结果显示该菌株与ATCC6051的相似性为98.7%,说明该菌为枯草芽孢杆菌(Bacillussubtilis)。
李雅婷应璐苏勇华蒋慧
关键词:骆驼刺枯草芽孢杆菌菌株特性
初花期骆驼刺与苜蓿混贮对降解效果的影响被引量:1
2011年
[目的]研究初花期骆驼刺与苜蓿混贮对降解效果的影响,为生产实践提供理论依据。[方法]以初花期骆驼刺、苜蓿为材料,采用人工瘤胃技术,分析体外降解率、产气量及降解液pH值、氨态氮(NH3-N)、挥发性脂肪酸(VFA)含量的变化,研究初花期骆驼刺和苜蓿混合青贮对降解效果的影响,并确定合适的骆驼刺与苜蓿混贮比例。[结果]与单一苜蓿相比,骆驼刺与苜蓿混合青贮后,显著降低了降解液中氨态氮含量,产气量有所下降,乙酸、丙酸和丁酸含量显著增加;骆驼刺70%+苜蓿30%的氨态氮含量和产气量较低,降解率、丙酸、丁酸、乙酸含量及三者总量较高,所以降解效果较好。[结论]初花期骆驼刺与苜蓿混贮,能提高苜蓿中蛋白质和碳水化合物的利用效率,骆驼刺70%+苜蓿30%的降解效果较好。
蒋慧应璐张秀萍陈宏伟马金萍
关键词:骆驼刺苜蓿混贮降解效果
Effect of Mixed Silage of Alhagi sparsifolia and Medicago sativa at Initial Flowering Stage on Degradation
2010年
[Objective] The influence of mixed silage of A.sparsifolia and M.sativa at initial flowering stage on degradation was studied to provide theoretical basis for production practice.[Method] Using A.sparsifolia and M.sativa at initial flowering stage as materials,the changes of in vitro degradation rate,gas production,pH value,ammoniacal nitrogen(NH3-N)and volatile fatty acid(VFA)content in degradation solution were analyzed by means of artificial rumen technique,and the effect of mixed silage of A.sparsifolia and M.sativa at initial flowering stage on degradation was studied in order to find out suitable mixed silage ratio of A.sparsifolia and M.sativa.[Result] Compared with single M.sativa,NH3-N content in degradation solution was very significantly decreased in the mixed silage of A.sparsifolia and M.sativa,and there was a reduction in gas production,while the content of acetic acid,propionic acid and butyric acid increased significantly;for the treatment of 70% A.sparsifolia and 30% M.sativa,NH3-N content(22.93 mmol/L)and gas production(40.50 ml)was lowest,while degradation rate(32.27%),acetic acid(5.48 mmol/L),propionic acid(2.08 mmol/L),butyric acid(2.52 mmol/L)and their total content(10.08 mmol/L)were highest,so its degradation effect was the best.[Conclusion] The mixed silage of A.sparsifolia and M.sativa at initial flowering stage could improve the utilization efficiency of protein and carbohydrate in M.sativa and the treatment of 70% A.sparsifolia and 30% M.sativa had the best degradation effect.
蒋慧应璐张秀萍陈宏伟马金萍
不同比例枯黄期骆驼刺与棉籽壳混贮对青贮品质的影响被引量:9
2010年
本试验旨在研究不同比例枯黄期骆驼刺与棉籽壳混贮对青贮品质的影响。7个处理(处理Ⅰ~Ⅶ)分别采用枯黄期骆驼刺、棉籽壳及其二者不同比例(3:7、4:6、5:5、6:4和7:3)混合料为青贮原料,每个处理3个重复。评价感官指标,并分析青贮料的pH、粗蛋白质(CP)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、乙酸、丙酸、丁酸、乳酸及总酸的变化。结果表明:骆驼刺虽然是豆科牧草,却容易青贮成功,青贮发酵以单乳酸发酵为主。处理Ⅴ、Ⅵ和Ⅶ中的可溶性碳水化合物含量显著高于处理Ⅱ(P<0.05),处理Ⅳ、Ⅴ、Ⅵ和Ⅶ的pH和氨态氮占总氮的百分比显著和极显著低于处理Ⅱ(P<0.05,P<0.01),处理Ⅳ、Ⅴ、Ⅵ和Ⅶ的总酸含量极显著高于处理Ⅱ(P<0.01)。混合青贮饲料中乳酸含量均极显著高于处理Ⅱ(P<0.01)。由此得出,骆驼刺与棉籽壳混贮能改善棉籽壳青贮料的品质。
蒋慧方雷张玲晋玉霞马春晖
关键词:骆驼刺棉籽壳青贮品质
枯黄期骆驼刺与稻草混贮对青贮饲料品质的影响被引量:27
2011年
采用枯黄期骆驼刺、稻草及其二者不同比例混合料为青贮原料,通过鉴定感官指标,分析青贮料的pH值、CP、NDF、ADF、乙酸、丙酸、丁酸、乳酸及总酸的变化,研究骆驼刺与稻草不同比例混贮对青贮品质的影响。结果表明,骆驼刺虽然是豆科牧草,却容易成功青贮,青贮发酵以乳酸发酵为主(乳酸/总酸大于50%);骆驼刺与稻草混贮,能显著提高稻草青贮原料的可溶性碳水化合物含量(P<0.05),降低pH值和NH3-N/TN的百分比(P<0.01),提高混贮料中乳酸,丙酸(P<0.01)和总酸(P<0.05)含量,骆驼刺占混贮料的40%以上时就能显著降低青贮料中丁酸(P<0.05)的含量,改善稻草的青贮品质。
蒋慧张玲马金萍晋玉霞万英马春晖
关键词:骆驼刺稻草青贮品质
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