Objective： In previous studies, Lactobacillus plantarum ZJ316 showed probiotic properties, such as an- timicrobial activity against various pathogens and the capacity to significantly improve pig growth and pork quality. The purpose of this study was to reveal the genes potentially related to its genetic adaptation and probiotic profiles based on comparative genomic analysis. Methods： The genome sequence of L. p/antarum ZJ316 was compared with those of eight L. plantarum strains deposited in GenBank. BLASTN, Mauve, and MUMmer programs were used for genome alignment and comparison. CRISPRFinder was applied for searching the clustered regularly interspaced short palin- dromic repeats （CRISPRs）. Results： We identified genes that encode proteins related to genetic adaptation and pro- biotic profiles, including carbohydrate transport and metabolism, proteolytic enzyme systems and amino acid bio- synthesis, CRISPR adaptive immunity, stress responses, bile salt resistance, ability to adhere to the host intestinal wall exopolysaccharide （EPS） biosynthesis, and bacteriocin biosynthesis. Conclusions： Comparative characterization of the L. plantarum ZJ316 genome provided the genetic basis for further elucidating the functional mechanisms of its probiotic properties. ZJ316 could be considered a potential probiotic candidate.
