Phosphate-dissolving bacteria isolated from rhizosphere of mangrove were characterized by 16S rDNA PCR-RFLP and 16S rDNA sequence to describe the genetic diversity and phylogeny. The dendrogram of 16S rDNA PCR-RFLP revealed the 24 isolated stains were assembled at 59% similarity level. The strains were grouped into 7 subgroups at 80% similarity. Phylogenetic tree of 16S rDNA sequences was constructed with representative strains in reference to the homogenous sequences in Genebank,and the result showed that Bacillus sp. was the dominant member,besides Paenibacillus sp.,Vibrio sp.,Klebsiella sp. and Pseudomonas sp. were also found.The highly genetic diversity was highly revealed by this study.In addition,the phosphate-dissolving capacity to dissolve solubilize the unsolvable inorganic phosphorus of bacteria was tested.All representative strains had the phosphate-dissolving capacity.The pH of liquid media of strains both were reduced to some degree,but the correlations were not found between phosphate-dissolving capacity and pH of the culture solution.