Perilla frutescens(labiatae),as a Chinese traditional and herbal drug with a long history,was determined to consist of abundant bioactive constituents,which make it a potential resource for pharmaceutical(utilization.) The ethyl acetate extract of fresh Perilla frutescens was tested to have a strong antibacterial activity.Four compounds were obtained by column chromatography.The results of antibacterial experiment show that the four compounds all have the activity for inhibiting growth of Staphylowccus aureas and Escherichia Coli.On the basis of chemical and spectral evidences,their chemical structures were determined as 3,3′-diethoxy rosmarinic acid(1),Luteolin(2),Caffeic acid(3), Rosmarinic acid(4).Compound 1 was novel.
