The Middle Jurassic palynomorphs from the Yan'an Formation in Dongsheng region of Nei Monggol, consist of 63 species of fossil spores and pollen grains belonging to 34 genera, of which two species were described as new. Based on statistical analysis of 3 863 specimens identified in 10 samples from Hantaichuan, Nianpanlianggou and Liugou sections, the sporo-pollen assemblage from the Yan'an Formation in Dongsheng region was established, which is generally characterized by the slight dominance of gymnospermous pollen grains (generally attaining proportion of 51%-54%), whereas the pteridophytic spores reach 46%-49% in abundance. In comparison with those of adjacent regions as well as Eurasia and North America, the present sporo-pollen assemblage is regarded to early Middle Jurassic (corresponding to Bajocian Stage) in geological age. According to the climatic conditions reflected by the palynoflora, the paleoclimate in Dongsheng region is suggested to be warm temperate or subtropical warm and humid during the early Middle Jurassic.