[Objective] The aim was to optimize experimental conditions of Gala apple NIR（Near Infrared）spectroscopy acquisition through study on the accuracy of NIR spectroscopy information under different influence factors.[Method]Gala apple was taken as the experimental material,and FieldSpec3 spectrometer（Analytical Spectral Device Company,America）was employed to acquire the NIR spectroscopy at room temperature.Then,the effects of different conditions including the environmental stray light,instrument stability,different distance,different chromatic aberration,different sites and different shelf-life on spectroscopy acquisition were investigated.[Result]The stray light had a significant effect on the visible light region of NIR spectroscopy;FieldSpec3 spectrometer had higher stability in 9 h;the measure distance of 2.5-12.5 mm could obtain good repeatability of absorbance;the effects of different chromatic aberration of the same apple on spectra was acceptable;the spectral stability of equatorial plane of the apple was better than those of the bottom and stalk;at room temperature;different shelf-life could make significant influence to NIR spectroscopy of the apple simple.[Conclusion]This study will provide reference for the researchers and analysis workers of apple NIR spectroscopy.
