A shell-and-tube heat exchanger with separated baffles parallel to segmental baffles was proposed, and an investigation on velocity distribution, heat transfer and flow resistance characteristics in the shell side of the proposed heat exchanger and traditional single segmental baffled shell-and-tube heat exchanger was reported. After the experiment, the empirical relations of heat transfer and flow resistance were obtained. The heat transfer enhancement comprehensive evaluation factor η was in the range from 1.15 to 1.20 for Reynolds number (1.25×10 4—3.35×10 4). The results indicated that flow field uniformization was enhanced in the shell side of shell-and-tube heat exchangers by two separated baffles parallel to segmental baffles and the performance of heat transfer was improved by controlling the dimension of vortices. The results could be used as reference in further research.