At the moment,little is found on the whole genomes of spiders in public databases.To this end,this study aimed to analyze the morphology,biological characteristics,and genome of Pardosa pseudoannulata,which was expected to add genome information of spiders.To be specific,Illumina sequencing was used to sequence the genome of this spider species,and K-mer(K=17)analysis to estimate the size,heterozygosity,and repetitiveness of the genome.More than 142.11 Gb high-quality reads were obtained,which were assembled into 3508.13 Mb contigs(N50=1035 bp)and 3542.56 Mb scaffolds(N50=1258 bp).Two peaks were found in the 17-mer distribution map.The genome of P.pseudoannulata was 4696.86 Mb,with the heterozygosity of 0.21%and 84.32%of repetitive sequences.This indicated that repetitive sequences were highly abundant in the genome of P.pseudoannulata and multi-generation selfing before genome sequencing can reduce the repetitiveness.In addition,NOVOheter can be used for genome assembly.This study layed a basis for the publication of spider genome data and provided a reference for the research on spider genomics.
