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核酸适体在生物医学分析中的应用: ＜正＞核酸适体（Aptamer）是通过指数富集配体系统进化技术（SELEX）,从DNA/RNA文库中筛选得到的能高亲和性和高特异性地与靶标分子结合的单链寡核苷酸。核酸适体可与细胞、生物大分子、蛋白质、有机分子、无机离子等...; 杨朝勇; 文献传递

不可逆封合微流控芯片中高活性蛋白质阵列的制备: 2012年; 保持生物分子的高活性是在不可逆封合微流控芯片中构筑微阵列芯片的关键问题.首先,利用MEMS技术和表面修饰方法制作了一种聚二甲基硅氧烷(PDMS)/玻璃芯片.应用光刻技术制作了PDMS盖片上的通道,同时用光刻剥离技术制作了玻璃基片上的金膜图案.进而,使用双官能团修饰剂3-氨丙基三甲氧基硅氧烷(APTMS)在玻璃基体和金膜图案上进行选择性表面修饰以吸附形成蛋白质阵列,并在其上覆盖一层水溶性聚乙烯醇(PVA)来保护蛋白质,既可避免其在加热处理过程中的高温伤害,又能防止在PDMS盖片与玻璃基片进行不可逆封合过程中的氧等离子体轰击造成的活性伤害.然后,通入水溶液冲洗除去PVA膜.使用荧光显微镜和原子力显微镜(AFM)考察蛋白质阵列质量,并结合免疫反应实验和细胞捕获固定实验评估蛋白质阵列的活性.结果表明,使用该方法可在不可逆封合的微流控芯片制作中构筑具有直径为200μm的高分辨率蛋白质阵列图案,蛋白质保持高的免疫活性,且可用于固定Hela细胞.; 周勇亮胡冬洁张大霄孙文; 关键词：微流控芯片蛋白质阵列

Allosteric Molecular Beacons for DNA Methyltransferase Detection: DNA methyltransferase(DAM)catalyzes the transfer of a memyl group from S-adenosylmethionine(SAM)to the 6-posit...; Weiting ZhangYanling SongXiaolong ZuZhu ZhiChaoyong Yang; 关键词：ALLOSTERIC DNA METHYLTRANSFERASE FLUORESCENT

A Surface Displaying Technology for Efficient Aptamer Selection: Aptamers are single-stranded oligonucleotides that can bind their target molecules with high affinity and spec...; Zhi ZhuYanling SongCong LiWeiting ZhangChaoyong James Yang; 关键词：SINGLE-MOLECULE EMULSION PCR APTAMER SELECTION

Microfluidic droplet-based single-molecule enzyme-linked immunosorbent assay for highly sensitivity digital detection of proteins: Proteins are essential components of organisms and are involved in a wide range of biological functions.Protei...; Mingxia ZhangZhichao GuanDongfeng ZhangZhi ZhuChaoyong James Yang; 关键词：DROPLET DIGITAL DETECTION PROTEIN

DNAzyme Linking Hydrogel for Portable Test of Lead Ion: Lead metal pollution in the environment may severely endanger the human health, especially the children.It is ...; Yishun HuangZhi ZhuChaoyong James Yang; 关键词：DNAZYMES HYDROGEL LEAD

循环取代酶切信号放大新技术及其在高灵敏SNP基因分型中的应用: ＜正＞核酸的高灵敏和高选择性检测在现代生命科学领域具有重要的意义。目前主要通过结合核酸酶进行信号放大的方法提高其检测灵敏度。然而,这种检测方法很难同时实现对核酸的高灵敏和高选择性检测。本文结合核酸外切酶Ⅲ（Exonucl...; 崔亮柯国梁王弛王春明吴崔晨林浩学杨朝勇; 文献传递

Target Induced Complementary Aptamer Fragments:A New Molecular Engineering Strategy for Light Switching Excimer Probes: ＜正＞The ability to detect or monitor important species in biological samples is of great importance to biomedic...; Chaoyong James Yang; 文献传递

Design and Synthesis of Photocleavable Caged MB: ＜正＞Light-activated compounds have been widely used as caging reagents in oligonucleotide synthesis for control...; Chaoyong James Yang; 文献传递

Facile and rapid generation of micro-collagen-gel array for high throughput long-term single cell 3D culture: Heterogeneity in cell populations po ses a major obstacle to understanding complex biological processes.The ne...; Shasha JiaZhichao GuanZhi ZhuChaoyong James Yang; 关键词：CULTURE THROUGHPUT

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国家重点基础研究发展计划(2010CB732402)