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Neurotensin expressed by glioma-initiating cells affects clinical outcome in glioma patients: Malignant glioma is the most common and fatal tumor in central nervous system,with the poor prognosis even wit...; Liang YI~1,Hualiang XIAO~2,Minhui XU~3,Tunan CHEN~1,Fei LI~1,Mei LI~1,Chunxia LUO~1,Mingyue ZHAO~1, Hua FENG~(1,*) 1 Department of Neurosurgery,Southwest Hospital,Third Military Medical University,Chongqing,China 2 Department of Pathology,Daping Hospital,Third Military Medical University,Chongqing,China 3 Department of Neurosurgery,Daping Hospital,Third Military Medical University,Chongqing,China; 关键词：NEUROTENSIN GLIOMA; 文献传递

纳络酮早期干预对大鼠弥漫性轴索损伤的影响被引量：1: 2010年; 目的观察纳络酮早期干预对大鼠实验性弥漫性轴索损伤(DAI)的治疗效果。方法采用改良Marmarou的方法制作大鼠颅脑DAI模型。将Wister雄性大鼠99只随机分为对照(假损伤)组、损伤组和干预组,干预组用纳络酮2mg/kg于伤后45min腹腔一次性注射给药,于伤后2、6、24、72h观察动物行为、脑组织含水量及组织病理学变化。结果损伤组大鼠伤后2、6、24、72h,行为学评分(满分为21.00±0.00分)分别为(9.05±1.52)、(12.12±1.41)、(17.23±1.34)和(19.36±0.92)分;干预组各时间点分别为(9.32±1.23)、(14.48±1.54)、(18.68±1.09)和(20.18±0.75)分;其中干预组伤后6和24h与损伤组比较差异显著(P<0.05)。对照组24h脑组织含水量测定结果为(78.19±0.35)%;伤后2、6、24、72h脑组织含水量,损伤组分别为(79.91±0.18)%、(80.70±0.49)%、(81.69±0.40)%和(80.32±0.42)%;干预组分别为(79.22±0.33)%、(79.59±0.46)%、(80.44±0.49)%和(79.50±0.44)%。同损伤组比较,干预组致伤6h后脑含水量均明显减轻(P<0.01)。干预组光镜下病理损害明显减轻。结论纳络酮伤后早期干预,有助于减轻大鼠DAI后继发性脑损害。; 张易储卫华陈景宇李梅张礼均吴国材冯华; 关键词：弥漫性轴索损伤纳络酮行为学评分脑含水量

Implantation of neurosphere derived from murine malignant glioma cell line GL261 into C57/BL6 mice:A reliable syngeneic graft model for research on glioma-initiating cells in vivo: Glioma-initiating cells(GICs) have been proven to play key roles in initiation and progress of malignant gliom...; Chun ZHOU,Tun-An CHEN,Liang YI,Min-Hui XU,Chun-Xia LUO,Fei LI,Mei LI,Jun HU,Ming-Yue ZHAO, Hua FENG~* Department of Neurosurgery,Southwest Hospital,Third Military Medical University,Chongqing,China; 关键词：GLIOMA NEUROSPHERE; 文献传递

Neurotensin expressed by glioma-initiating cells affects clinical outcome in glioma patients: Objective Malignant glioma is the most common and fatal tumor in central nervous system, with the poor prognos...; Liang Yi1 ,2 Minhui Xu1 Hua Feng2 1.Department of Neurosurgery, Daping Hospital, Third Military Medical University, Chongqing, 4 0004 2 , China 2.Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 4 0004 2 , China; 关键词：NEUROTENSIN GLIOMA; 文献传递

Neurotensin expressed by glioma-initiating cells affects clinical outcome in glioma patients: ＜正＞Malignant glioma is the most common and fatal tumor in central nervous system,with the poor prognosis even ...; 易良; 文献传递

纳络酮早期干预对大鼠弥漫性轴索损伤后延髓髓鞘的作用观察: 2011年; 目的观察纳络酮(Naloxone)干预对弥漫性轴索损伤(diffuse axonal injury,DAI)病理损害的影响。方法采用改良的Marmarou大鼠颅脑DAI模型。Wistar雄性大鼠(99只),随机分为对照(假损伤)、损伤、纳络酮干预(2mg/kg于伤后45分腹腔一次性注射)3个组,于伤后2、6、24、72小时作行为学评分并观察病理形态学变化。结果大鼠DAI后神经功能障碍即时发生,干预组与损伤组比较,6小时及24小时评分有显著差异(P<0.05)。神经纤维嗜银染色切片光镜下见,损伤组24小时病理损害程度最重,延髓纤维排列紊乱,回缩球大量形成,干预组中轴突走行较正常,回缩球密度小。电镜观察可见损伤组24小时延髓髓鞘板层明显分离甚或轴索断裂,干预组轴索断裂少见。结论早期、大剂量纳络酮干预可减轻大鼠DAI后延髓髓鞘继发性病理损害。; 张易储卫华陈景宇李梅张礼均吴国材冯华; 关键词：轴索损伤纳络酮

Glioma-initiating cells:a predominant role in microglia/macrophages tropism to glioma: The relationship between cancer-initiating cells and cancer-related inflammation is unclear.Exploring the inte...; Liang YI~1,Hualiang XIAO~2,Minhui XU~3,Xianzong YE~2,Jun HU~1,Fei LI~1,Mei LI~1,Chunxia LUO~1,Shicang YU~2, Xiuwu BIAN~2,Hua FENG~(1,*) 1 Department of Neurosurgery,Southwest Hospital,Third Military Medical University,Chongqing,China 2 Institute of Pathology,Southwest Hospital,Third Military Medical University,Chongqing,China 3 Department of Neurosurgery,Daping Hospital,Third Military Medical University,Chongqing,China; 关键词：MICROGLIA GLIOMAS; 文献传递

高压氧对脑内种植GL261胶质瘤C57小鼠尼莫司汀化疗后生存期的影响被引量：4: 2012年; 目的观察高压氧辅助治疗对脑内种植GL261胶质瘤细胞系C57小鼠尼莫司汀(嘧啶亚硝脲)化疗后平均生存期的影响。方法于C57小鼠右侧尾状核种植GL261胶质瘤细胞,术后第7天分别接受单纯高压氧或单次尼莫司汀化疗或高压氧与尼莫司汀联合治疗,观察不同治疗方法对荷瘤鼠生存期的影响。结果大体标本观察显示,不同处理组荷瘤鼠肿瘤体积均较大、占位效应明显,正常脑组织受压严重、中线结构偏移,但不同处理组肿瘤体积比较差异无统计学意义(F=0.602,P=0.618)。平均生存期(F=12.177,P=0.000)评价和Kaplan-Meier生存曲线分析(χ2=13.604,P=0.003)显示,单纯高压氧治疗(χ2=0.365,P=0.546)和单次尼莫司汀化疗(χ2=0.884,P=0.347)对荷瘤鼠生存期无明显影响;高压氧联合尼莫司汀化疗(χ2=9.962,P=0.002)可延长荷瘤鼠生存时间,且疗效优于单纯高压氧治疗(χ2=6.925,P=0.008)和单次尼莫司汀化疗(χ2=7.152,P=0.007)。结论高压氧对颅内种植GL261胶质瘤细胞系的C57小鼠生存期和生长无明显影响和促进作用,联合尼莫司汀化疗则可使荷瘤鼠平均生存期明显延长,提示高压氧对尼莫司汀化疗具有增效作用。; 兰川李飞陈图南叶信珍吴南易良林江凯朱刚冯华; 关键词：高压氧神经胶质瘤尼莫司汀肿瘤种植

人原代胶质瘤起始细胞的生物学行为初步研究被引量：1: 2012年; 目的研究人原代胶质瘤起始细胞(GICs)的表型、自我更新能力、成瘤能力和耐药性等生物行为学特性。方法运用常规培养法和条件培养法对来自手术切除的同一胶质母细胞瘤组织分别进行培养,同时获得贴壁生长的人原代胶质瘤细胞(AGCs)和GICs;通过免疫荧光染色法测定其神经胶质酸性蛋白(GFAP)、CD133和巢蛋白的表达,通过单克隆形成实验和梯度密度成瘤实验检测其成球和成瘤能力,利用细胞计数药盒-8检测两种细胞在嘧啶亚硝脲(ACNU)和长春新碱(VCR)作用下的增殖曲线。结果 GICs呈神经球样生长且表达CD133和巢蛋白;47.8%±5.6%的GICs能够自我更新,而可表达GFAP的AGCs中仅有4.3%±1.6%能形成单克隆;ACNU对GICs的IC_50=1×10^(-3.1)mol/L,对AGCs的IC_50=1×10^(-3.9)mol/L,两者相比较,相差显著(P<0.05);VCR对GICs的IC_50=1×10^(-4.3)mol/L,对AGCs的IC_50=1×10^(-5.5)mol/L,两者相比较,亦相差显著(P<0.05)。结论利用条件培养法可以获得具有明显干性的GICs,与分化的AGCs相比具有更强的自我更新能力和成瘤能力,且GICs对ACNU和VCR较AGCs更为耐药。; 陈图南易良李飞赵明月蔡云王晖黄磊冯华; 关键词：干细胞标志物自我更新成瘤能力化疗抵抗

Implantation of neurosphere derived from murine malignant glioma cell line GL261 into C57/BL6 mice: A reliable syngeneic graft model for research on glioma-initiating cells in vivo: Objective Glioma-initiating cells (GICs) have been proven to play key roles in initiation and progress of mali...; Liang Yi1 ,2 Minhui Xu1 Hua Feng2 1.Department of Neurosurgery, Daping Hospital, Third Military Medical University, Chongqing, 4 0004 2 , China 2.Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 4 0004 2 , China; 关键词：GLIOMA; 文献传递

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