Objective To study single wall carbon nanotubes （SWCNT） and its role in inducing inflammatory cytokines in the cruor-fibrinolysis system of rat. Methods Twenty one Wistar rats were divided into four groups： 1） control; 2） low-dose SWCNT （0.15 mg/kg BW）; 3） medium-dose SWCNT （0.75 mg/kg BW）; 4） high-dose SWCNT （1.5 mg/kg BW）. Intratracheal instillation of SWCNT suspensions was administered to rats once per day for 21 days. In order to assess the exposure effect of SWCNT to the rats, activity of Inflammatory cytokine was measured and markers of cruor-fibrinolysis system were studied via ELSIA. Also, change in clotting time was recorded and histopathology was studied. Results IL-6 and IL-8 concentrations of rats exposed to SWCNT were significantly higher than those in controls （P〈0.05）. The activity of inflammatory cytokines and histopathological change indicated that oxidative damage occurred. Change in clotting time in rats exposed to SWCNT decreased compared with controls. Meanwhile, t-PA （tissue-tupe plassminogen activator） and AT-Ⅲ （antithrombin-Ⅲ） levels in rats exposed to particulates increased or decreased significantly compared with controls （P〈0.05）. A similar trend was observed for D-dimer （D2D） levels, indicating that SWCNT can impact the cruor-fibrinolysis system ofrat. Conclusion The results from our study suggest that an increased procoagulant activity and reduced fibrinolytic activity in rats exposed to SWCNT can cause pulmonary oxidative stress and inflammation, due to the release of pro-thrombotic and inflammatory cytokines into the blood circulation of rat.
TIAN LeiLIN Zhi QingLIN Ben ChengLIU Huan LiangYAN JunXI Zhu Ge
