[Objective] The study aimed to better understand the effect of different fertilizer treatments on micro-morphological characteristics of a purple soil at the 0-20 cm topsoil in a long-term fertilizer experiment. [Method] Soil micro-morphology was observed and analyzed under a single polarizing microscope. [Result] For the CK (no fertilizer) treatment, soil structure was dense with little porosity developed. Its soil microstructure was poor, sandy fabric-granular fabric. After continuously applied chemical fertilizers only for more than two decades, the soil particles did not evolve into soil structures and formed little porosity. The microstructures of soil in N, NP and NPK treatments were porphyroskelic fabric-fine sandy granular fabric, better than that of the soil in CK treatment. Adding manure obviously improved the quantity of groundmass and endowed the soil a loose structure and plenty porosity, enriched animal and plant residues, and well-formed iron-manganese nodules and humus ma- terials, all resulting in better micro-aggregates development. The type of soil microstructures in MNPK treatment was pectized-compacted takyric fabric-intertextic fabric, the best among all the treatments. [Conclusion] Combined application of both or- ganic and inorganic fertilizers can significantly improve the structure of the purple soil, enhance soil fertility and achieve soil sustainable development.