High-throughput SNP detection microarrays were used here to explore the relationship between 5, 10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphism C677T and the risk of gastric carcinoma among population in Jiangsu region,by genotyping the specimens from 170 patients with gastric carcinoma and 140 age-and sex- matched control subjects.PCR products were spotted onto a 3-aminopropyltriethoxysilane coated glass slide to fabricate a microarray,then interrogated by hybridization with dual-color probes (Cy3,CyS) to determine the SNP genotype of each sample,and the relation between the genotypes and the risk of gastric carcinoma was analyzed.The frequencies of C677T genotype were CC(47.9%),CT(40%),CT(12.1%) in control group and CC(35.9%),CT(45.9%),TT(18.2%) in gastric carcinoma group,respectively.The individuals with 677CT+TT genotype group or 677TT had a 1.67-fold (95% CI:1.06-2.64) or 2.67-fold (95% CI:1.382-5.341) increased risk to develop gastric carcinoma compared with those having 677CC genotype. It was shown that the single nucleotide polymorphisms in the MTHFR gene are associated with the risk of gastric carcinoma in the Chinese population.
A novel maskless technique, self-driving micro-fluid porous type printing （SMPTP）, was reported to in situ synthesize oligonucleotide arrays on glass slide, which has the merits of low cost, high quality and simple craft. In SMPTP for fabricating gene- chips, porous fiber tubes with a number of nanometric or micron channels functioned as ＂active letters＂ and were assembled in designed patterns, which are identical to the distribution of monomers in each layer of the array, and four patterns were needed for each layer. By means of capillarity, the synthesis solution was automatically taken into porous tubes assembled in a printing plate and reached the surface. An oligonucleotide array of 160 features with four different 15-mer probes was in situ synthesized using this technique. The four specific oligonucleotide probes, including the matched and the mismatched by the fluorescent target sequence, gave obviously different hybridization fluorescent signals.
