目的探索即刻早期抗原(immediate early antigen,IE抗原)检测在骨髓移植术后人巨细胞病毒感染中的诊断价值。方法回顾性分析骨髓移植术后160份外周血标本,根据外周血巨细胞病毒被膜抗原pp65的不同分为感染组和对照组,运用免疫组化法检测外周血人巨细胞病毒IE抗原和被膜抗原pp65。结果20名患者中,实验组16名(136份血标本),对照组4名(24份血标本)。160份外周血标本中,人巨细胞病毒pp65抗原阳性标本95份(59.38%),IE抗原阳性94份(56.75%)(P=0.91);IE抗原和pp65抗原术后检出时间分别为2.44±1.38周和3.17±1.16周(P=0.031)。结论IE抗原检测能发现早期特异性反应HCMV感染。
BACKGROUND: Liver transplantation (LT) is an effective therapy for end-stage hepatitis B virus (HBV) infection. Recurrence of HBV is one of the frequent complications. In the present study, we investigated whether human leukocyte antigen (HLA) matching influences the incidence of HBV recurrence, and the time point of HBV recurrence after LT. METHODS: One hundred and two recipients of LT with end-stage chronic HBV infection were reviewed. The triple-drug immunosuppression regimen consisted of tacrolimus, mycophenolate, and prednisone. All patients were subjected to prophylaxis with hepatitis B immunoglobulin and lamivudine. HLA typing was performed using a sequence-specific primer-polymerase chain reaction kit. Serology for hepatitis B and HBV DNA was examined using a commercial kit. RESULTS: The incidence of recurrent HBV infection post-LT was 6.86%. The recurrent infection of HBV was independent of the degree of H LA matching (P>0.05). The time point of HBV recurrence, however, was prolonged in HLA-A matched patients compared with matchless patients (P=0.049). The recurrence of HBV infection was independent of H LA compatibility. CONCLUSIONS: This retrospective analysis showed that more HLA-A locus compatibility is associated with a prolonged time of recurrence of HBV in patients after LT for end-stage HBV infection. The incidence of HBV recurrence is independent of HLA compatibility. (Hepatobiliary Pancreat Dis Int 2010; 9: 139-143)
