Huntington’s disease(HD)is a genetic disease characterized by the progressive degeneration of the striatum and cortex.Patients can present with a variety of symptoms that can broadly be classified into motor symptoms,inclusive of choreatic movements and rigidity,mood and psychiatric symptoms,such as depression and apathy,and cognitive symptoms,such as cognitive decline.The causal mutation underlying HD results from an expansion of a CAG repeat sequence on the IT15 gene,resulting in the formation and accumulation of a mutant huntingtin protein.
Pathological tremor is one of the cardinal symptoms in Parkinson's disease (PD).Tremor is comprised of involuntary,rhythmic,a nd oscillating movements that can vary according to the circumstances under which they occur,the body parts that are involved,and the frequency at which they present.For example,tremors can be mild to severe,are stress sensitive,and can affect arms,legs,or the head (Dirkx and Bologna,2022).
[Objectives]This study was conducted to investigate the alleviating effect of Phellodendri Chinensis Cortex extract on the phytotoxicity of pretilachlor in rice.[Methods]In addition to CK,Phellodendri Chinensis Cortex extract and pretilachlor with different gradients were added to the culture medium,and rice seeds with the same bud length were evenly placed in the culture medium.After 10 and 15 d of culture,the plant height and fresh weight of rice seedlings were measured and the alleviation rate was calculated.[Results]Ten days after application,the plant height of treatment 8(pretilachlor:extract concentration ratio of 25:200)was alleviated with an alleviation rate of 21.72%,and the fresh weight of treatment 9(pretilachlor:extract concentration ratio of 25:400)was alleviated with an alleviation rate of 31.04%.Fifteen days after treatment,the plant height of treatment 6(pretilachlor:extract concentration ratio of 25:50)showed a better alleviating effect,and the fresh weight of treatment 8(pretilachlor:extract concentration ratio of 25:200)exhibited a better alleviating effect,with an alleviation rate of 22.39%.Meanwhile,it was found that the extract of Phellodendri Chinensis Cortex could alleviate the increase of POD activity in rice leaves caused by pretilachlor.Meanwhile,it was found that the extract of Phellodendri Chinensis Cortex promoted the expression of CAT in rice seedlings,thus significantly increasing its activity and alleviating the toxicity of pretilachlor to rice.[Conclusions]This study can provide technical support for the screening and field application of plant-derived safeners.
Lycii Radicis Cortex(LRC)is a medicinal and food homologous plant with various pharmacological activities,including anti-tumor effects.This study explores the anti-tumor effect of LRC on non-small cell lung cancer(NSCLC)and its molecular mechanism using mice bearing Lewis lung carcinoma cells.LRC significantly suppressed the growth of NSCLC.Besides,RNA sequencing of mice tumors and hematoxylin&eosin and immunofluorescence staining revealed that LRC promoted the infiltration of T lymphocytes,specifically GZMB~+CD8~+T lymphocytes,in tumor tissues.The Gene Set Enrichment Analysis of spleen RNA indicated that LRC up-regulated PD-1-downstream pathways,suggesting that LRC exerted its effects through the PDL1/PD-1 pathway.Further experiments revealed that LRC interacted with PD-L1,blocking PD-L1/PD-1 binding and thus restoring the T cell killing activity on tumor cells.Together,these results support using LRC as healthy food to improve anti-tumor immunity in patients with NSCLC.
